Investigation of tumour microenvironment in influencing growth in lobular breast cancers

Closing date: 09/03/2026

MB-PhD Studentship: Investigation of tumour microenvironment in influencing growth in lobular breast cancers

Lead Supervisors: Dr Sankari Nagarajan
Co-Supervisors:
Dr Sumitra Mohan, Prof. Robert Clarke, Dr Andrew Gilmore, Dr Ciara O’ Brien

Applications Deadline: Monday 9th March 2026
Interviews: Week commencing 27th April 2026
Start date: September 2026

Project Keywords: Lobular breast cancer, fibroblasts, epigenetics
Research Opportunity: MB-PhD Studentship

Project Outline

Lobular breast cancers represent one of the understudied cancers but of unmet need, due to their poor response to targeted therapies. Failure in drug response is often associated with differential molecular changes in cancer cells according to the origin of the tumour (ductal or lobular), either associated with DNA sequences (genetic) or other than DNA that affect gene expression (so-called epigenetic alterations). Understanding the coupled genetic and transcriptional reprogramming is critical in revealing the key regulators of treatment response. However, this interactive reprogramming is poorly understood in any cancer, let alone lobular cancers. Moreover, our current knowledge on these cancers includes evidence accumulated from in vitro-based studies which don’t properly replicate the patient samples.

This project aims to understand the previously unknown lobular-specific transcriptional reprogramming influenced by the stromal landscape, by studying gene alterations and gene expression using 3D models, in vivo xenografts and primary tumour tissues. Our study will explore the following:

  • Identify the influence of stromal factors on lobular cancer growth
  • Categorise the primary lobular cancer samples with ARID1A expression (loss or presence)
  • Investigate the mutation-specific stromal and immune landscape and its role in gene and protein expression reprogramming

Importantly, our comparative analysis with lobular and ductal samples will allow us to identify mechanisms of differential growth response and transcriptional reprogramming, which can indicate potential effective therapeutic approaches.

The proposed study will utilise a multidisciplinary supervisory team with lot of training opportunities for students in 3D culturing, in vivo assays, spatial imaging and spatial transcriptomics profiling. We successfully obtained access to lobular and ductal tumour samples with matched FFPE and frozen material from Breast Cancer Now Tissue biobank for this study. This will be an unbiased one-of-a-kind study investigating the interactive transcriptional reprogramming in controlling growth response.

Applications for this project are now open. Please complete your application on The University of Manchester Postgraduate Application Portal.

About Dr Sankari Nagarajan (project Lead Supervisor)

I am a breast cancer research scientist investigating the transcriptional regulation of disease progression in breast cancers. I completed my Integrated M.Sc. in Bio-Medical Science at Bharathidasan University, Trichy, India. After obtaining a prestigious and competitive PhD scholarship from German Academic Exchange Service (DAAD), I moved to the University of Goettingen, Germany, to work with Prof. Dr. Steven A Johnsen, for my PhD. 

I am currently the deputy director of Manchester Breast Centre, scientific committee member of Lobular Breast Cancer UK charity and the co-lead of Divisional Research Community and culture team. I strongly support patient engagement activities and mentors early career students/postdocs to support women in academia and science with childcare responsibilities.  

Find out more

Headshot of Prof. Sankari Nagarajan

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